WWOX Knockout cell line (iPSC)

WWOX Knockout cell line (iPSC) Order

Catalog#

YKO-H224

Size

2*10^5

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

WWOX knockout cell line (iPSC)

Morphology

Spherical clone

Passage ratio

1:6-1:8

Species

Human

Gene Name

WWOX

Gene ID

51741

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

Target region

Knockout region: Exon 4 (based on transcript WWOX-214)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:iPSC			STR Info (Cell bank) Cell Line:ATCC- DYR0100 hiPSC
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	11	13		11	13
D7S820	9	10		9	10
D16S539	12	12		12	12
VWA	14	19		14	19
TH01	9.3	9.3		9.3	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	7	11		7	11

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the WWOX gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the iPSC cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, WWOX gene knockout WWOX knockout cell line (iPSC) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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WWOX Knockout cell line (iPSC)

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